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KMID : 0355220080330030247
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Journal of Korean Academy Oral Medicine
2008 Volume.33 No. 3 p.247 ~ p.256
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Expression of Clusterin in the Salivary Gland under Restraint Stress
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Park Hee-Kyung
Hong Jung-Pyo
Auh Q-Schick
Chun Yang-Hyun
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Abstract
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The belief that stress leads to illness has a long history. A number of the orofacial disease are also closely associated with stress. Despite research in the relationship of stress and the orofacial diseases leading to statistically significant correlations, the pathology remains vague. In the present study, the expression of clusterin, a stress protein responsible for antiapoptosis and cytoprotection, under restraint stress condition was observed in the submandibular gland, one of the major salivary glands. Sprague-Dawley rats were divided into 2 groups: normal group and restraint stress group. The rats of restraint stress group were placed in the stress cages and then sacrificed at day 0, 3 and 5 day of the experiment. After that, the submandibular glands of all the rats were excised immediately. The levels of clusterin proteins and mRNA in the tissues were measured by immunohistochemistry and Northern blot analyses, respectively. The results were as follows:
1. In the immunohistochemistry, clusterin protein was detected only immediately after the application of restraint stress.
2. In the restraint stress group, at day 3 and 5, histologically apoptosis was induced with karyorrhectic and pyknotic changes.
3. By the restraint stress, acinic cells were destructed earlier than ductal cells.
4. In the Northern blot, mRNA of clusterin was expressed only immediately after the application of restraint stress.
The overall results suggest that as an early response to stress, clusterin is expressed in the glands to protect the glandular cells from the stress. But if stress is so strong and prolonged that it can exceed the stress adaptability of the cells, then the cells may undergo apoptosis instead of producing clusterin. An Epidemiologic Study of Symptoms of Temporomandibular Disorders in Korean College Students.
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KEYWORD
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Restraint Stress, Salivary Gland, Clusterin, mRNA
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